Study of quality markers of antiuric acid formula by grey relational analysis

Abstract Gout has become a global problem, antiuric acid formula (AAF) is clinical prescription highly effective in reducing uric levels. In order to find its quality control standards and contribute the treatment of gout future, we adopted high-performance liquid chromatography orbitrap chromatography–mass spectrometry establish fingerprints 13 batches AAF. The different AAF were tested activity inhibit by xanthine oxidase inhibition experiment. Grey relational analysis bio-activity validation assess spectrum–effect relationship. Finally, choose puerarin, calycosin-7- O -beta- d -glucoside puerarin apioside as component, average content 6036.006 μg/g, 296.113 μg/g 878.285 μg/g. As components AAF, can be great significance for related research.